8GCK

Crystal structure of the human CHIP-TPR domain in complex with a 6mer acetylated tau peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.116
Spacegroup name	P 2 21 21
Unit cell lengths	45.745, 65.717, 77.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.745 - 1.368
R-factor	0.193268926362
Rwork	0.192
R-free	0.20994
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.842
Data reduction software	xia2
Data scaling software	xia2
Phasing software	BALBES
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.750	1.417
High resolution limit [Å]	1.368	1.368
Number of reflections	49457	4695
<I/σ(I)>	7.61
Completeness [%]	99.2	94.96
Redundancy	11.8
CC(1/2)	0.996	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M CaCl2, 0.1 M HEPES pH 7.4, 25% PEG 3350