8GBQ
Structure of RNF125 in complex with UbcH5b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 56.181, 56.181, 164.347 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.870 - 1.740 |
R-factor | 0.1848 |
Rwork | 0.184 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.650 | 48.650 | 1.770 |
High resolution limit [Å] | 1.740 | 9.040 | 1.740 |
Rmerge | 0.062 | 0.042 | 1.263 |
Rmeas | 0.064 | 0.044 | 1.310 |
Rpim | 0.017 | 0.012 | 0.342 |
Total number of observations | 3214 | 20365 | |
Number of reflections | 59049 | 289 | 1537 |
<I/σ(I)> | 21.7 | 50.6 | 2 |
Completeness [%] | 99.4 | 98.8 | 89 |
Redundancy | 14.7 | 11.1 | 13.2 |
CC(1/2) | 0.999 | 0.998 | 0.687 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 0.1 M CHES 9.5 20% PEG 8000 |