8GBN
Structure of Apo Human SIRT5 P114T Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.017, 114.896, 56.265 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 41.020 - 2.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.020 | 2.797 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.612 |
| Number of reflections | 13273 | 1062 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 92.3 | |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.5, 20% PEG 10K, 10 mM Praseodymium (III) acetate hydrate |
