8G2D
Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.70A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-05 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.360, 449.770, 616.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 254.370 - 2.700 |
R-factor | 0.2268 |
Rwork | 0.224 |
R-free | 0.27730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.838 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 254.370 | 254.370 | 2.770 |
High resolution limit [Å] | 2.700 | 12.070 | 2.700 |
Rmerge | 0.163 | 0.042 | 1.159 |
Rmeas | 0.186 | 0.048 | 1.356 |
Total number of observations | 6279665 | ||
Number of reflections | 1527540 | 16763 | 109267 |
<I/σ(I)> | 7.32 | 21.99 | 0.99 |
Completeness [%] | 97.2 | 90.2 | 94.3 |
Redundancy | 4.111 | 4.591 | 3.538 |
CC(1/2) | 0.983 | 0.994 | 0.205 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |