8G2C
Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-27 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.650, 448.120, 621.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 162.050 - 2.650 |
R-factor | 0.2092 |
Rwork | 0.207 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhw |
RMSD bond length | 0.004 |
RMSD bond angle | 0.824 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 162.050 | 162.050 | 2.720 |
High resolution limit [Å] | 2.650 | 11.850 | 2.650 |
Rmerge | 0.124 | 0.023 | 1.203 |
Rmeas | 0.146 | 0.027 | 1.416 |
Total number of observations | 5736096 | ||
Number of reflections | 1649189 | 18690 | 122695 |
<I/σ(I)> | 8.49 | 31.88 | 1.02 |
Completeness [%] | 98.7 | 94.6 | 99.6 |
Redundancy | 3.478 | 3.593 | 3.516 |
CC(1/2) | 0.994 | 0.999 | 0.253 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |