8G29

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	210.090, 449.740, 625.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.520 - 2.550
R-factor	0.2154
Rwork	0.213
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.835
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	153.520	153.520	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.136	0.027	1.728
Rmeas	0.152	0.030	1.922
Total number of observations	9731340
Number of reflections	1877927	21935	138438
<I/σ(I)>	10.57	43.49	0.97
Completeness [%]	99.1	98.1	99.1
Redundancy	5.182	5.296	5.395
CC(1/2)	0.995	0.998	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME