8G29

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution

This is a non-PDB format compatible entry.

Summary for 8G29

• Entry DOI: 10.2210/pdb8g29/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (61 entities in total)
• Functional Keywords: cfr, methyltransferase, multidrug, antibiotic, resistance, methylation, a2503, 23s rrna, 70s ribosome, inhibition of translation, peptidyl transferase center, nascent peptide exit tunnel, iboxamycin, tylosin, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4562737.93

Authors

Aleksandrova, E.V.,Wu, K.J.Y.,Tresco, B.I.C.,Syroegin, E.A.,Killeavy, E.E.,Balasanyants, S.M.,Svetlov, M.S.,Gregory, S.T.,Atkinson, G.C.,Myers, A.G.,Polikanov, Y.S. (deposition date: 2023-02-03, release date: 2023-12-27, Last modification date: 2024-01-31)

• Primary citation: Aleksandrova, E.V.,Wu, K.J.Y.,Tresco, B.I.C.,Syroegin, E.A.,Killeavy, E.E.,Balasanyants, S.M.,Svetlov, M.S.,Gregory, S.T.,Atkinson, G.C.,Myers, A.G.,Polikanov, Y.S.; Structural basis of Cfr-mediated antimicrobial resistance and mechanisms to evade it.; Nat.Chem.Biol., 2024; PubMed: 38238495; DOI: 10.1038/s41589-023-01525-w

Experimental method

X-RAY DIFFRACTION (2.55 Å)