8G18
Heterodimer of the GluN1b-GluN2B NMDA receptor amino-terminal domains bound to allosteric inhibitor 93-108
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9198 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 269.172, 60.076, 145.572 |
| Unit cell angles | 90.00, 117.02, 90.00 |
Refinement procedure
| Resolution | 34.370 - 2.850 |
| R-factor | 0.17782 |
| Rwork | 0.174 |
| R-free | 0.24579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qel |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.527 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.073 | 0.776 |
| Number of reflections | 48988 | 489 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | sodium formate, HEPES, sodium chloride, Tris |
