8FZU
The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Thr-Val linker variant, Expressed with SUMO tag
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 |
Unit cell lengths | 164.096, 164.096, 54.401 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 71.060 - 1.900 |
R-factor | 0.2392 |
Rwork | 0.238 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.660 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.060 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.179 | 2.574 |
Rmeas | 2.709 | |
Rpim | 0.059 | |
Number of reflections | 66252 | 2186 |
<I/σ(I)> | 4.67 | |
Completeness [%] | 90.5 | |
Redundancy | 10.1 | |
CC(1/2) | 0.998 | 0.607 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M HEPES 2.0M ammonium sulfate |