8FZU

The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Thr-Val linker variant, Expressed with SUMO tag

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	164.096, 164.096, 54.401
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.060 - 1.900
R-factor	0.2392
Rwork	0.238
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.060	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.179	2.574
Rmeas		2.709
Rpim	0.059
Number of reflections	66252	2186
<I/σ(I)>	4.67
Completeness [%]	90.5
Redundancy	10.1
CC(1/2)	0.998	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1M HEPES 2.0M ammonium sulfate