8FZ3

Sterile Alpha Motif of Human Translocation ETS Leukemia, Non-Polymer Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 65
Unit cell lengths	61.506, 61.506, 166.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.840 - 2.784
R-factor	0.2306
Rwork	0.229
R-free	0.26650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qar
RMSD bond length	0.007
RMSD bond angle	0.838
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.840	2.883
High resolution limit [Å]	2.784	2.784
Rmerge	0.125	1.480
Rmeas	0.128	1.516
Rpim	0.029	0.330
Number of reflections	8929	898
<I/σ(I)>	20
Completeness [%]	99.8	100
Redundancy	20	21
CC(1/2)	1.000	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	298	1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic pH 8.2