8FX0
Crystal structure of the Trypanosoma cruzi hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRT), isoform D, bound to (S)-SerMe-ImmH Phosphonate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2018-04-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.199, 80.706, 90.228 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.080 - 1.520 |
R-factor | 0.2085 |
Rwork | 0.208 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tc2 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.738 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.080 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rpim | 0.118 | 0.957 |
Number of reflections | 66957 | 3287 |
<I/σ(I)> | 13.6 | 4.6 |
Completeness [%] | 97.1 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M lithium sulfate, 24% PEG3350, 0.1 M HEPES, pH 8.0 |