8FSE
Crystal structure of integrin beta-2 tail bound to the FERM-folded talin head domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92010 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.862, 110.358, 68.368 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 47.780 - 1.900 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.798 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.133 | 0.432 |
| Number of reflections | 77701 | 3617 |
| <I/σ(I)> | 8.4 | 1.1 |
| Completeness [%] | 99.3 | 94.7 |
| Redundancy | 3.2 | 2.2 |
| CC(1/2) | 0.963 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | PEG4000 16%, NaCl 0.1M, DTT 2mM |
