8FR3
E. coli EF-Tu in complex with KKL-55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.385, 65.222, 70.559 |
| Unit cell angles | 107.67, 98.79, 107.08 |
Refinement procedure
| Resolution | 64.820 - 2.230 |
| R-factor | 0.2464 |
| Rwork | 0.245 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eze |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.820 | 2.310 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.110 | 0.882 |
| Rmeas | 0.121 | 0.967 |
| Rpim | 0.050 | 0.392 |
| Number of reflections | 42498 | 3782 |
| <I/σ(I)> | 9.79 | |
| Completeness [%] | 94.0 | 84.31 |
| Redundancy | 5.9 | 5.8 |
| CC(1/2) | 0.994 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30-35% polyethylene glycol monomethyl ether 5,000 (PEG 5K MME), 0.2 M (NH4)2(SO4), 0.1 M MES pH 6.5 |
