8FPX

Crystal structure of tumor related RhoA mutant A161P in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97931
Spacegroup name	P 1 21 1
Unit cell lengths	32.212, 87.265, 34.044
Unit cell angles	90.00, 95.91, 90.00

Refinement procedure

Resolution	31.570 - 1.470
R-factor	0.1935
Rwork	0.191
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftn
RMSD bond length	0.006
RMSD bond angle	0.930
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.570	1.550
High resolution limit [Å]	1.470	1.470
Rmeas	0.286	1.075
Rpim	0.148	0.640
Number of reflections	31430	4322
<I/σ(I)>	3	1
Completeness [%]	98.9	93.4
Redundancy	3.6	2.7
CC(1/2)	0.930	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0