8FPX
Crystal structure of tumor related RhoA mutant A161P in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.212, 87.265, 34.044 |
| Unit cell angles | 90.00, 95.91, 90.00 |
Refinement procedure
| Resolution | 31.570 - 1.470 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.930 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.570 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmeas | 0.286 | 1.075 |
| Rpim | 0.148 | 0.640 |
| Number of reflections | 31430 | 4322 |
| <I/σ(I)> | 3 | 1 |
| Completeness [%] | 98.9 | 93.4 |
| Redundancy | 3.6 | 2.7 |
| CC(1/2) | 0.930 | 0.446 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0 |
