8FNX

Crystal structure of Hyaluronate lyase B from Cutibacterium acnes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-05-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	103.676, 103.676, 172.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.730 - 2.100
R-factor	0.1577
Rwork	0.156
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.823
Data reduction software	XDS (VERSION Nov 1, 2016 BUILT=20170215)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.730	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.172	0.672
Rmeas	0.178	0.699
Rpim	0.047	0.188
Number of reflections	55667	7843
<I/σ(I)>	14.5	4.3
Completeness [%]	99.5	97.8
Redundancy	14.1	13.1
CC(1/2)	0.997	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.2 M Na dihydrogen phosphate pH 6.5, 9% PEG 8000, 5 mM TCEP