8FJ0
Crystal Structure of the Tick Evasin EVA-AAM1001(Y44A) Complexed to Human Chemokine CCL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.446, 49.636, 88.319 |
| Unit cell angles | 90.00, 112.91, 90.00 |
Refinement procedure
| Resolution | 43.610 - 2.910 |
| R-factor | 0.2554 |
| Rwork | 0.254 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.700 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.110 | 3.070 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 14772 | 2250 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 98.5 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.971 | 0.475 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2 M NH4SO4, 0.1 M bis-tris, Ph 5.6 |
