8FBZ
Crystal Structure of apo human Glutathione Synthetase Y270E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-03 |
Detector | DECTRIS PILATUS3 S 2M |
Wavelength(s) | 1.0 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 80.834, 94.051, 125.122 |
Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
Resolution | 47.070 - 1.590 |
Rwork | 0.160 |
R-free | 0.18880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.357 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.070 | 1.630 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.093 | 0.687 |
Rmeas | 0.106 | 0.778 |
Rpim | 0.066 | 0.359 |
Number of reflections | 123489 | 5993 |
<I/σ(I)> | 10.1 | 2.1 |
Completeness [%] | 98.6 | |
Redundancy | 4.3 | |
CC(1/2) | 0.996 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | HEPES, PEG400, ammonium sulfate |