8FBH
Crystal structure of NSD1 Mutant-T1927A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.293, 67.382, 70.025 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.170 - 2.320 |
| R-factor | 0.1908 |
| Rwork | 0.188 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ooi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 6.290 | 2.320 |
| Rmerge | 0.111 | 0.054 | 0.937 |
| Rmeas | 0.119 | 0.057 | 1.035 |
| Rpim | 0.042 | 0.020 | 0.428 |
| Total number of observations | 110550 | ||
| Number of reflections | 13958 | 782 | 695 |
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.8 | 98.7 | 99.7 |
| Redundancy | 7.9 | 7.6 | 5.7 |
| CC(1/2) | 0.998 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2.1M NH4SO4, 0.2M NaCl, 0.1M Hepes pH |
