8F6M
Complex of Rabbit muscle pyruvate kinase with ADP and the phosphonate analogue of PEP mimicking the Michaelis complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.026, 216.396, 258.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.020 - 2.150 |
R-factor | 0.1584 |
Rwork | 0.156 |
R-free | 0.20470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g50 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.112 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.127 | 0.046 | 0.583 |
Total number of observations | 2026691 | ||
Number of reflections | 545313 | 54458 | 54585 |
<I/σ(I)> | 7.9 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15 mM succinate, 50 mM KCl, 19 to 24% PEG 8,000 well solution |