8F5U
Rabbit muscle pyruvate kinase in complex with magnesium, potassium and pyruvate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 113.131, 130.198, 170.282 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.270 - 2.300 |
R-factor | 0.1667 |
Rwork | 0.164 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g50 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.746 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.960 | 2.300 |
Rmerge | 0.140 | 0.061 | 0.541 |
Total number of observations | 810287 | ||
Number of reflections | 216125 | 21594 | 21593 |
<I/σ(I)> | 7.8 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 3.7 | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 282 | 50 mM Tris 50 mM NaCl and a range of 7.5 to 10% PEG 20,000 |