8F59
LSD1-CoREST in complex with AW2 and SNAG peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-23 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9654 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 120.206, 179.201, 236.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.730 - 2.800 |
| R-factor | 0.2209 |
| Rwork | 0.221 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.085 | 1.535 |
| Rmeas | 0.096 | 1.754 |
| Rpim | 0.043 | 0.828 |
| Total number of observations | 277195 | 19524 |
| Number of reflections | 62710 | 4567 |
| <I/σ(I)> | 10.1 | 1.1 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 0.997 | 0.309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Na/K Tartrate, 100 mM ADA pH 6.5 |
