8F27

Mirror-image DNA containing 2'-OMe-L-uridine residue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	99
Detector technology	CCD
Collection date	2022-10-15
Detector	RAYONIX MX-300
Wavelength(s)	0.987
Spacegroup name	P 65
Unit cell lengths	46.310, 46.310, 43.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.510 - 1.600
R-factor	0.19306
Rwork	0.193
R-free	0.20517
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7moo
RMSD bond length	0.026
RMSD bond angle	3.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.510	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.044	0.559
Rmeas	0.048	0.694
Rpim	0.020	0.402
Number of reflections	7021	470
<I/σ(I)>	22.1	2.2
Completeness [%]	99.4	93.6
Redundancy	9.8	4.8
CC(1/2)	1.000	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.5 M Lithium sulfate, 50 mM TRIS pH 8.5, 5 % w/v Glycerol