8F24

Mirror-image RNA octamer containing 2'-OMe-L-uridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	99
Detector technology	CCD
Collection date	2022-10-15
Detector	RAYONIX MX-300
Wavelength(s)	0.9787
Spacegroup name	H 3 2
Unit cell lengths	47.070, 47.070, 365.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.940 - 3.200
R-factor	0.2215
Rwork	0.213
R-free	0.29824
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kw4
RMSD bond length	0.019
RMSD bond angle	2.501
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.310
High resolution limit [Å]	2.270	2.270
Rmerge	0.074	0.874
Rmeas	0.077	0.903
Rpim	0.021	0.224
Number of reflections	2795	709
<I/σ(I)>	30.1	4.2
Completeness [%]	98.5	99.7
Redundancy	8.2	8.4
CC(1/2)	0.983	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.0 M Ammonium sulfate, 0.1 M HEPES sodium pH 7.5, 2% v/v Polyethylene glycol 400