8EW6

Anti-human CD8 VHH complex with CD8 alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-10-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00001
Spacegroup name	C 1 2 1
Unit cell lengths	57.115, 83.554, 51.302
Unit cell angles	90.00, 98.48, 90.00

Refinement procedure

Resolution	32.481 - 1.904
R-factor	0.2024
Rwork	0.200
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NULL
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.048	0.041	0.254
Rmeas	0.058	0.049	0.316
Rpim	0.032	0.027	0.186
Total number of observations	48809
Number of reflections	16891	956	636
<I/σ(I)>	11.4
Completeness [%]	90.9	99.9	69.5
Redundancy	2.9	3.3	2.4
CC(1/2)		0.994	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	15% PEG 3350 and 0.1M succinic acid pH 7.0