Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 125.342, 125.342, 58.084 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.275 - 2.559 |
| Rwork | 0.224 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x7j |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.249 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.275 | 2.630 |
| High resolution limit [Å] | 2.559 | 2.559 |
| Rmerge | 0.072 | 1.180 |
| Number of reflections | 17214 | 1254 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4% tacsimate pH 7.0 and 12% PEG 3350 |
