8EQ5

Crystal structure of the N-terminal kinase domain of RSK2 in complex with SPRED2 (131-160)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979180
Spacegroup name	I 1 2 1
Unit cell lengths	83.438, 41.105, 99.258
Unit cell angles	90.00, 113.87, 90.00

Refinement procedure

Resolution	49.390 - 1.800
R-factor	0.185
Rwork	0.183
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gue
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.771
Rpim	0.057	0.752
Number of reflections	28136	1656
<I/σ(I)>	8.8
Completeness [%]	97.7
Redundancy	3.3
CC(1/2)	0.990	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	30% w/v PEG 2K MME, 0.1 M Tris, pH 8.0