8EO2

Lufaxin a bifunctional inhibitor of complement and coagulation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	64.480, 71.310, 167.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.180 - 2.310
R-factor	0.2039
Rwork	0.201
R-free	0.25500
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.180	42.180	2.370
High resolution limit [Å]	2.310	10.330	2.310
Rmerge	0.057	0.034	0.553
Rmeas	0.060	0.036	0.592
Total number of observations	302907
Number of reflections	34638	455	2524
<I/σ(I)>	21.15	40.85	4.12
Completeness [%]	99.9	98.7	99.9
Redundancy	8.745	7.187	8.004
CC(1/2)	0.999	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% PEG 6000, 100 mM Tris HCl, pH 7.0