8ENK

Crystal structure of UAP56 in complex with Tho1, the yeast homolog of human SARNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	88.534, 96.786, 126.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 2.500
R-factor	0.2253
Rwork	0.224
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xtj
RMSD bond length	0.003
RMSD bond angle	0.695
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.071	0.918
Rpim	0.031	0.396
Number of reflections	38274	1873
<I/σ(I)>	28.7	1.9
Completeness [%]	99.9
Redundancy	6.2
CC(1/2)	0.999	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.025 M HEPES, pH 7.4, 0.2 M sodium chloride, and 18% PEG4000