8EKF
X-ray crystal structure of 311R Fab in complex with the PfCSP peptide NPNA-3
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.412, 70.765, 170.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.270 - 1.900 |
| Rwork | 0.184 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6axk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.718 |
| Data reduction software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.270 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | |
| Number of reflections | 44216 | 4800 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.8 | 97.6 |
| Redundancy | 3.3 | 2.3 |
| CC(1/2) | 0.990 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.04 M KH2PO4, 20% Glycerol, and 16% PEG3000 |
