8EKB

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA, deacylated P-site tRNAmet, and thermorubin at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.510, 449.730, 622.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	155.540 - 2.700
R-factor	0.2112
Rwork	0.209
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.868
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	155.540	155.540	2.770
High resolution limit [Å]	2.700	12.080	2.700
Rmerge	0.166	0.044	1.774
Rmeas	0.184	0.049	1.971
Total number of observations	8236656
Number of reflections	1538795	17421	112047
<I/σ(I)>	8.87	32.03	0.99
Completeness [%]	97.0	92.9	95.9
Redundancy	5.353	5.59	5.184
CC(1/2)	0.993	0.996	0.177

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME