8EK8
Human PU.1 ETS-Domain (165-270) Bound to d(AATAAAAGGAGAAGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000050 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.058, 61.064, 154.255 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.810 - 2.630 |
R-factor | 0.241 |
Rwork | 0.237 |
R-free | 0.27970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.003 |
RMSD bond angle | 0.580 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.810 | 2.724 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.075 | 0.936 |
Rmeas | 0.031 | 0.374 |
Rpim | 0.081 | 1.000 |
Number of reflections | 12962 | 1263 |
<I/σ(I)> | 17.17 | 2.43 |
Completeness [%] | 98.7 | 99.21 |
Redundancy | 7 | 7.1 |
CC(1/2) | 0.999 | 0.926 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 200 mM Ammonium Acetate, 100 mM TRIS, pH=8.5, 25% PEG 3350 |