8EK6

UCA Y35N (unbound) Fab from CH65-CH67 lineage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	124.547, 70.707, 86.934
Unit cell angles	90.00, 126.96, 90.00

Refinement procedure

Resolution	49.760 - 1.954
Rwork	0.185
R-free	0.21590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hk0
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.900	1.950
Rmerge		0.356
Rmeas	0.052	0.503
Number of reflections	43266	6868
<I/σ(I)>	17.23	1.93
Completeness [%]	98.3
Redundancy	2
CC(1/2)	0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M succinic acid (pH 7), 0.1 M bicine (pH 8.5), 30% v/v polyethylene glycol monomethyl ether 550