8EK5
Engineered scFv 10LH bound to PHOX2B/HLA-A24:02
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.087, 174.562, 47.193 |
Unit cell angles | 90.00, 118.75, 90.00 |
Refinement procedure
Resolution | 43.680 - 2.110 |
R-factor | 0.18319 |
Rwork | 0.181 |
R-free | 0.21245 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7mja |
RMSD bond length | 0.008 |
RMSD bond angle | 1.296 |
Data reduction software | XDS (Jan 10,2022) |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHENIX (1.19.2-4158) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.680 | 2.115 |
High resolution limit [Å] | 2.108 | 2.108 |
Rmerge | 0.095 | 0.644 |
Rmeas | 0.111 | 0.776 |
Rpim | 0.057 | 0.389 |
Number of reflections | 37369 | 377 |
<I/σ(I)> | 4.5 | 2.2 |
Completeness [%] | 97.4 | 98.7 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.995 | 0.817 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.2M Sodium Nitrate |