8EJP
Crystal structure of the homeodomain of Platypus sDUX in complex with DNA containing 5-Bromouracil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.499, 74.499, 99.688 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.676 - 2.174 |
R-factor | 0.2397 |
Rwork | 0.238 |
R-free | 0.25930 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.676 | 59.676 | 2.329 |
High resolution limit [Å] | 2.174 | 7.091 | 2.174 |
Rmerge | 0.112 | 0.091 | 1.444 |
Rmeas | 0.117 | 0.095 | 1.504 |
Rpim | 0.032 | 0.026 | 0.416 |
Total number of observations | 139973 | 6026 | 6739 |
Number of reflections | 10466 | 523 | 523 |
<I/σ(I)> | 10.7 | 21 | 1.5 |
Completeness [%] | 94.4 | 99.6 | 64.5 |
Redundancy | 13.4 | 11.5 | 12.9 |
CC(1/2) | 0.998 | 0.997 | 0.649 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350 |