8EJ8

Human PU.1 ETS-Domain (165-270) Q226E Mutant Bound to d(AATAAAAGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	42.960, 60.611, 44.659
Unit cell angles	90.00, 116.73, 90.00

Refinement procedure

Resolution	33.320 - 1.450
R-factor	0.1383
Rwork	0.136
R-free	0.17160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.008
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.320	1.502
High resolution limit [Å]	1.450	1.450
Rmerge	0.051	0.464
Rmeas	0.061	0.549
Rpim	0.033	0.290
Number of reflections	34842	3396
<I/σ(I)>	13.9	2.38
Completeness [%]	95.8	94.12
Redundancy	3.4	3.5
CC(1/2)	0.997	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350