8EHO
PRRSV-1 PLP2 domain bound to ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-09-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5406 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.686, 159.492, 147.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.940 - 2.850 |
| R-factor | 0.21408 |
| Rwork | 0.212 |
| R-free | 0.24658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PDB-REDO (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.840 | 9.010 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 24728 | 3640 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 97.1 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.987 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 3350, 0.2M Mg(NO3)2 |
