8EHN
PRRSV-1 PLP2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.499, 102.347, 100.739 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.580 - 2.300 |
| R-factor | 0.20534 |
| Rwork | 0.200 |
| R-free | 0.25296 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.440 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.580 | 8.910 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.042 | |
| Number of reflections | 15677 | 1516 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 22% PEG 2000, 0.35M ammonium acetate, 0.1M HEPES 7.5 |
