8EFG

Crystal structure of human TATDN1 bound to dAMP and two zinc ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127230
Spacegroup name	P 21 21 21
Unit cell lengths	55.880, 76.510, 77.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.300 - 1.500
R-factor	0.164
Rwork	0.163
R-free	0.18880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xio
RMSD bond length	0.011
RMSD bond angle	1.542
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	1.600
High resolution limit [Å]	1.500	1.505
Rmeas	0.060	0.743
Number of reflections	52962	8656
<I/σ(I)>	15.93	3.02
Completeness [%]	99.4	92.7
Redundancy	6.62	6.7
CC(1/2)	0.999	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	19% PEG 8000, 100 mM HEPES pH 7.0