8EEC

Crystal structure of HPK1 citron-homology domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-07-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	81.667, 81.667, 140.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.614 - 2.500
R-factor	0.2108
Rwork	0.208
R-free	0.25670
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	Aimless (0.2.8)
Phasing software	AutoSol
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.850	46.850	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge	0.156	0.039	1.390
Rmeas	0.163	0.041	1.446
Rpim	0.045	0.013	0.395
Total number of observations	218660	6822	32537
Number of reflections	17174	649	2449
<I/σ(I)>	18.6	57.7	2.1
Completeness [%]	100.0	99.6	100
Redundancy	12.7	10.5	13.3
CC(1/2)	0.998	0.999	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.4 M ammonium phosphate