8EE5

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ119-D in complex with ZIKV E glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97
Spacegroup name	P 21 2 21
Unit cell lengths	51.681, 103.672, 196.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.994 - 3.583
R-factor	0.2513
Rwork	0.249
R-free	0.29640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nip
RMSD bond length	0.003
RMSD bond angle	0.680
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.400	3.720
High resolution limit [Å]	3.580	3.580
Rmeas	0.210
Number of reflections	23586	1900
<I/σ(I)>	9.97
Completeness [%]	98.6
Redundancy	6.6
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.06M Nitrate Phosphate Sulfate , 0.1M Sodium HEPES and MOPS (acid) pH 7.5, 20% Ethylene glycol, 10 % PEG 8000 + 2% w/v Benzamidine hydrochloride