8EDJ

Crystal structure of rA3G-ssRNA-GA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	55.305, 68.058, 129.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.027 - 1.830
R-factor	0.1689
Rwork	0.168
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uu4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	1.830	1.830
Rmerge	0.085
Number of reflections	83087	4301
<I/σ(I)>	21.01
Completeness [%]	99.9
Redundancy	12.7
CC(1/2)	0.999	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.18 M Na2SO4, 20% PEG 3350