Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 85.550, 85.550, 265.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.070 - 2.130 |
| Rwork | 0.223 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7uli |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.070 | 2.364 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 17134 | 857 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 91.6 | |
| Redundancy | 18.2 | |
| CC(1/2) | 0.996 | 0.341 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M ammonium sulfate, 0.1 M HEPES, 35% w/v poly(acrylic acid sodium salt) 2100 |
