8E8T
Structure of the short LOR domain of human AASS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92010 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.356, 154.715, 70.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.910 - 2.180 |
| R-factor | 0.2152 |
| Rwork | 0.214 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qrj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.560 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.910 | 42.910 | 2.250 |
| High resolution limit [Å] | 2.180 | 8.990 | 2.180 |
| Rmerge | 0.172 | 0.079 | 1.089 |
| Rmeas | 0.206 | 0.096 | 1.319 |
| Rpim | 0.110 | 0.052 | 0.724 |
| Total number of observations | 132605 | 1976 | 10951 |
| Number of reflections | 43284 | 645 | 3757 |
| <I/σ(I)> | 4.4 | 11.8 | 1 |
| Completeness [%] | 96.7 | 90.4 | 98 |
| Redundancy | 3.1 | 3.1 | 2.9 |
| CC(1/2) | 0.981 | 0.984 | 0.367 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 0.2M NaCl, 0.1M HEPES, 25% PEG 4000 |
