8E6N
X-ray structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G223W mutant in an outward-open, manganese-bound state
Replaces: 6BU5Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 105.758, 80.389, 51.754 |
Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
Resolution | 39.190 - 2.400 |
R-factor | 0.2262 |
Rwork | 0.224 |
R-free | 0.27060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6c3i |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.190 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.186 | |
Rmeas | 0.210 | |
Rpim | 0.095 | |
Number of reflections | 13962 | 621 |
<I/σ(I)> | 6.2 | 1.6 |
Completeness [%] | 82.1 | 36.4 |
Redundancy | 3.5 | 1.7 |
CC(1/2) | 0.980 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6 | 298 | 50 mM succinic acid, 10 mM spermidine, 5 mM manganese chloride, 100 mM MES, 28% PEG400 |