8E2P
Crystal structure of TadA*8.20 in a complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.670, 84.670, 214.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.340 - 2.720 |
| R-factor | 0.197 |
| Rwork | 0.190 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z3a |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 2.790 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Number of reflections | 24824 | 1776 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.9 | |
| CC(1/2) | 0.995 | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3,350, ammonium acetate, TRIS pH 8.5 |
