8DZM
Crystal structure of human Sar1a in complex with ppGpp and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.125, 35.933, 76.118 |
| Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
| Resolution | 45.415 - 1.648 |
| R-factor | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f6b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.640 | 1.648 |
| Rmeas | 0.085 | 0.683 |
| Number of reflections | 43805 | 2066 |
| <I/σ(I)> | 18.9 | 1.15 |
| Completeness [%] | 99.5 | 95.3 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG6000 (w/v), 1.0M LiCl, 0.1M MgCl2, 0.1M sodium acetate buffer, pH4.5 |
