8DYS
Crystal structure of human Eukaryotic translation initiation factor 2A (eIF2A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.289, 84.847, 99.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.680 - 1.800 |
| R-factor | 0.2009 |
| Rwork | 0.200 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wj9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.436 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.680 | 47.640 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.108 | 0.066 | 0.808 |
| Rmeas | 0.117 | 0.072 | 0.864 |
| Rpim | 0.043 | 0.029 | 0.304 |
| Total number of observations | 2618 | 19679 | |
| Number of reflections | 42642 | 412 | 2481 |
| <I/σ(I)> | 13.5 | 25.3 | 3.6 |
| Completeness [%] | 98.8 | 99.4 | 98.1 |
| Redundancy | 7.7 | 6.4 | 7.9 |
| CC(1/2) | 0.995 | 0.994 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG 2K, 0.2M Potassium Bromide |
