8DY1

Crystal Structure of scFv CAT2200 LH in complex with IL-17A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	51.930, 62.050, 111.710
Unit cell angles	90.00, 99.69, 90.00

Refinement procedure

Resolution	43.690 - 2.680
R-factor	0.2231
Rwork	0.221
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qhu
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.690	43.690	2.750
High resolution limit [Å]	2.680	11.990	2.680
Rmerge	0.066	0.024	0.522
Rmeas	0.080	0.029	0.620
Number of reflections	18382	234	1341
<I/σ(I)>	13.09	29.91	2.57
Completeness [%]	92.2	95.1	91.8
Redundancy	3.22
CC(1/2)	0.996	0.999	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	298	100 mM Tris, 200 mM LiSO4, 18% PEG 3350