8DSW

Crystal structure of EGFR kinase domain, Exon20 Insertion FQEA mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-11
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	I 2 3
Unit cell lengths	148.555, 148.555, 148.555
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.980 - 2.390
R-factor	0.1973
Rwork	0.192
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2itx
RMSD bond length	0.008
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.980	2.450
High resolution limit [Å]	2.390	2.390
Rmerge		1.245
Number of reflections	20688	1966
<I/σ(I)>	16.6
Completeness [%]	95.0
Redundancy	7.4
CC(1/2)	0.998	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M potassium fluoride 20 % PEG 3350