8DSK

Structure of the N358Y variant of serine hydroxymethyltransferase 8 in complex with PLP, glycine, and formyl tetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	87.106, 90.798, 147.441
Unit cell angles	90.00, 90.55, 90.00

Refinement procedure

Resolution	74.680 - 1.630
R-factor	0.186
Rwork	0.184
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxj
RMSD bond length	0.007
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.800	1.650
High resolution limit [Å]	1.630	1.630
Rmerge	0.088	1.336
Rmeas	0.102	1.656
Rpim	0.052	0.801
Number of reflections	281201	12043
<I/σ(I)>	8.1	0.8
Completeness [%]	97.8	84.9
Redundancy	3.8	3.6
CC(1/2)	0.997	0.381

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.18-0.25 triethylamine oxide, 0.1 M Tris, pH 8.5, 21-23 % w/v PEG MME 2000